Method of validating analysing data
CPO analysis by GC is required for the appreciation of variability of its fatty acids composition among parents and progeny in view of creating improved oil palm breeding materials that will produce oil of better quality .
For precise quantitative analysis, it is essential to calibrate the GC–FID system using calibration standards for each analyte.
esterification, transesterification etc; • Instrument configuration and method development: e.g.
GC injector, oven and detector temperature settings, gas pressure and flow rate, retention time definition etc.
In this system, only one standard material is required and the cost for analysis is greatly reduced compared to the conventional calibration method using calibration standards for each analyte .
The four critical components for a method are: sampling, sample preparation, instrument analysis conditions and standardization.
Standard Method Development and Validation Parameters 4.
Sampling, Choice of Materials and Sample Preparation 3.
Conclusion Acknowledgement References The variety of available techniques and analytical methods applied in laboratories calls for the need of defining the scope, range of application and quality of information obtained using each of them with reference to internationally agreed standards.
Gas Chromatography seems to be the most appropriate method for qualitative and quantitative analysis of isolated lipid extracts.
Analytical processes for oils like palm oil often use Gas chromatography (GC) with flame ionization detector (GC–FID) method with derivatization. is made up of mainly triglycerides with some mono and diglycerides.
During the preliminary method development stage, all individual components should be investigated before the final method optimization.
This gives the scientist a chance to critically evaluate the method performance in each component and streamline the final method optimization .